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Image Search Results
Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: Nanoscale coupling of junctophilin-2 and ryanodine receptors regulates vascular smooth muscle cell contractility
doi: 10.1073/pnas.1911304116
Figure Lengend Snippet: Jph2 knockdown has no effect on Ca 2+ spark frequency, amplitude, or kinetics. ( A ) Representative confocal Ca 2+ images of pressurized (60 mmHg), Fluo-4-AM–loaded cerebral arteries treated with control or Jph2 -targeting morpholinos. Colored boxes show selected ROIs where Ca 2+ sparks occurred. (Scale bar, 10 µm.) ( B ) Representative changes in fractional fluorescence ( F / F 0 ) as a function of time for ROIs in A . The trace color corresponds to the color of the respective ROI box. ( C ) Summary data showing the Ca 2+ spark frequency (in Hertz) normalized to surface area (in Hertz per 100 square micrometers) in cerebral arteries ( n = 5 to 6 cerebral arteries/group from 4 animals), as well as the amplitude ( F / F 0 ), half-duration [half-time ( t 1/2 ), in seconds], rise time ( t 1/2 , in seconds), and decay time ( t 1/2 , in seconds) of individual Ca 2+ spark events recorded from each group ( n = 616 events for control, n = 601 events for Jph2 -targeted). There were no significant differences.
Article Snippet: Rows were successively loaded with Wes antibody diluent blocking buffer;
Techniques: Fluorescence